Gene Cloning

Gene Cloning PDF
Author: David M. Glover
Publisher: Springer
ISBN: 1489932461
Size: 32.88 MB
Format: PDF
Category : Medical
Languages : un
Pages : 222
View: 7666

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This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Molecular Cloning

Molecular Cloning PDF
Author: Joseph Sambrook
Publisher:
ISBN: 9780879695767
Size: 40.44 MB
Format: PDF, Docs
Category : Eukaryotic cells
Languages : un
Pages : 2000
View: 4546

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MolecularCloning.com contains summarized versions of protocols from the third edition of Molecular Cloning: A Laboratory Manual, published in December 2000. The first release of MolecularCloning.com contains protocols from the first of the three print volumes. In addition, the site contains a moderated bulletin board. The abbreviated protocols can be searched by keyword, downloaded, and printed out. The references cited within each protocol are linked to the National Library of Medicine's PubMed database (www.ncbi.nlm.nih.gov/PubMed) where abstracts of the papers can be consulted and links made to the full text of papers if available.

Guide To Molecular Cloning Techniques

Guide to Molecular Cloning Techniques PDF
Author: Shelby L. Berger
Publisher:
ISBN: 9780121817756
Size: 74.37 MB
Format: PDF, Mobi
Category : Science
Languages : un
Pages : 812
View: 3698

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Guide to Molecular Cloning Techniques is a self-contained, state-of-the-art manual designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Key Features * Comprehensive protocols compiled specifically for this volume * Among the topics covered * Genomic cloning * Preparation and characterization of mRNA * cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods

Molecular Cloning Of Hormone Genes

Molecular Cloning of Hormone Genes PDF
Author: Joel F. Habener
Publisher: Springer Science & Business Media
ISBN: 1461248248
Size: 56.15 MB
Format: PDF, Kindle
Category : Science
Languages : un
Pages : 442
View: 6493

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The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono cistronic mRNA appears to be a genetic mechanism for the gener ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc ture and expression of hormone-encoding genes.

Vectors

Vectors PDF
Author: Raymond L. Rodriguez
Publisher: Butterworth-Heinemann
ISBN: 1483103080
Size: 68.91 MB
Format: PDF, Docs
Category : Science
Languages : un
Pages : 592
View: 2187

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Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.

Molecular Cloning

Molecular Cloning PDF
Author: Michael Richard Green
Publisher:
ISBN:
Size: 55.52 MB
Format: PDF, ePub, Docs
Category : Molecular cloning
Languages : un
Pages : 1936
View: 2183

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Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. [...] The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.

Molecular Cloning

Molecular Cloning PDF
Author: Gregory G. Brown
Publisher: BoD – Books on Demand
ISBN: 9533073985
Size: 72.72 MB
Format: PDF, Mobi
Category : Medical
Languages : en
Pages : 338
View: 2382

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The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.

The Condensed Protocols From Molecular Cloning A Laboratory Manual

The Condensed Protocols from Molecular Cloning   a Laboratory Manual PDF
Author: Joseph Sambrook
Publisher: CSHL Press
ISBN: 0879697717
Size: 15.41 MB
Format: PDF, Mobi
Category : Science
Languages : un
Pages : 800
View: 4423

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The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.

Molecular Biology Of Plants

Molecular Biology of Plants PDF
Author: Russell Malmberg
Publisher: CSHL Press
ISBN: 9780879691844
Size: 25.93 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 150
View: 1688

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Molecular Cloning And Gene Regulation In Bacilli

Molecular Cloning and Gene Regulation in Bacilli PDF
Author: A. T. Ganesan
Publisher:
ISBN:
Size: 17.18 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 359
View: 2100

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Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid ...

Gene Cloning And Dna Analysis

Gene Cloning and DNA Analysis PDF
Author: T. A. Brown
Publisher: John Wiley & Sons
ISBN: 1119072565
Size: 31.94 MB
Format: PDF, Kindle
Category : Science
Languages : un
Pages : 376
View: 3680

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Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis: In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes New material on the use of ChiP-seq to locate protein-binding sites Extended coverage of the strategies used to assemble genome sequences Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.