Molecular Cloning

Molecular Cloning PDF
Author: Gregory G. Brown
Publisher: BoD – Books on Demand
ISBN: 9533073985
Size: 23.52 MB
Format: PDF, ePub
Category : Medical
Languages : en
Pages : 338
View: 6307

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The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.

Molecular Cloning

Molecular Cloning PDF
Author: Joseph Sambrook
Publisher:
ISBN:
Size: 36.38 MB
Format: PDF, ePub
Category : Eukaryotic cells
Languages : en
Pages : 1699
View: 1189

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Molecular Cloning

Molecular Cloning PDF
Author: Michael Richard Green
Publisher:
ISBN: 9781936113415
Size: 32.10 MB
Format: PDF, Mobi
Category : Medical
Languages : en
Pages : 1936
View: 3451

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Rev. ed. of: Molecular cloning: a laboratory manual / Joseph Sambrook, David W. Russell. 2001.

Molecular Cloning

Molecular Cloning PDF
Author: Tom Maniatis
Publisher:
ISBN:
Size: 60.80 MB
Format: PDF, ePub, Mobi
Category : Eukaryotic cells
Languages : en
Pages : 545
View: 5067

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Molecular Biology And Biotechnology

Molecular Biology and Biotechnology PDF
Author: John M. Walker
Publisher: Royal Society of Chemistry
ISBN: 9780854046065
Size: 56.90 MB
Format: PDF, Kindle
Category : Science
Languages : en
Pages : 563
View: 992

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This volume provides a revised and updated introduction to the techniques of molecular biology and its industrial applications. It should be of particular benefit to undergraduates and researchers in other biological areas.

Guide To Molecular Cloning Techniques

Guide to Molecular Cloning Techniques PDF
Author: Shelby L. Berger
Publisher:
ISBN: 9780121817756
Size: 61.63 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 812
View: 5156

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Guide to Molecular Cloning Techniques is a self-contained, state-of-the-art manual designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Key Features * Comprehensive protocols compiled specifically for this volume * Among the topics covered * Genomic cloning * Preparation and characterization of mRNA * cDNA cloning * Screening libraries * Identification and characterization of clones * Methods written by 87 experts * Editors' notes, overviews, and extensive cross-referencing * Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility * A problem-solving quantitative approach * Emphasis on strategies for choosing alternative methods

The Condensed Protocols From Molecular Cloning A Laboratory Manual

The Condensed Protocols from Molecular Cloning   a Laboratory Manual PDF
Author: Joseph Sambrook
Publisher: CSHL Press
ISBN: 0879697717
Size: 71.95 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 800
View: 2248

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The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.

Molecular Biology

Molecular Biology PDF
Author: Philip C. Turner
Publisher: Garland Science
ISBN: 9780415351676
Size: 57.11 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 370
View: 1035

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This book "includes: material on microRNAs and RNA inhibition ; a new section on functional genomics, proteomics, imaging, and stem cells ; an introduction to bioinformatics." -- back cover.

Molecular Cloning Of Hormone Genes

Molecular Cloning of Hormone Genes PDF
Author: Joel F. Habener
Publisher: Springer Science & Business Media
ISBN: 1461248248
Size: 67.32 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 442
View: 5324

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The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono cistronic mRNA appears to be a genetic mechanism for the gener ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc ture and expression of hormone-encoding genes.

Molecular Cloning And Gene Regulation In Bacilli

Molecular Cloning and Gene Regulation in Bacilli PDF
Author: A Ganesan
Publisher: Elsevier
ISBN: 0323153097
Size: 50.51 MB
Format: PDF, Mobi
Category : Nature
Languages : en
Pages : 382
View: 1817

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Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.

Vectors

Vectors PDF
Author: Raymond L. Rodriguez
Publisher: Butterworth-Heinemann
ISBN: 1483103080
Size: 17.64 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 592
View: 2974

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Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors. The book first discusses bacterial plasmid pBR322. Topics include criteria for plasmid vector design, construction and structure, transcriptional signals, DNA replication, recombination, mobilization, and plasmid stability. The text also examines bacteriophage lambda cloning vectors; filamentous phages as cloning vectors; chimeric single-stranded DNA phage-plasmid cloning vectors; and phage-plasmid hybrid vectors. The selection discusses cosmids and plasmid positive selection vectors, including library and construction, cosmid rescue, and positive selection vectors using plasmid-encoded lethal function. The text also examines vectors for regulating expression of cloned DNA, including lambda promoters, secretion vectors, and protein fusion vectors. The book takes a look at vectors with adjustable copy numbers. Copy number and protein production; adjustable copy number vectors; future expression vectors; rate-limiting steps of protein production; and promoters and ribosome binding sites are explained. The text puts emphasis on vectors for the synthesis of specific RNAs in vitro and cloning vectors for gram-positive bacteria. The selection is a valuable source of data for readers interested in molecular cloning vectors.

A History Of Molecular Biology

A History of Molecular Biology PDF
Author: Michel Morange
Publisher: Harvard University Press
ISBN: 9780674001695
Size: 50.46 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 336
View: 5779

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Gene therapy, the human genome project, the creation of new varieties of animals and plants have all emanated from molecular biology. Beginning with turn-of-the-century experimentations, this ambitious history covers the story of the transformation of biology over the last 100 years.

Protein Protein Interactions

Protein protein Interactions PDF
Author: Erica Golemis
Publisher: CSHL Press
ISBN: 0879697237
Size: 42.95 MB
Format: PDF, ePub, Docs
Category : Science
Languages : en
Pages : 938
View: 1635

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Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.