Handbook Of Single Molecule Biophysics

Handbook of Single Molecule Biophysics PDF
Author: Peter Hinterdorfer
Publisher: Springer Science & Business Media
ISBN: 9780387764979
Size: 80.57 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 626
View: 3386

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This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Handbook Of Single Molecule Fluorescence Spectroscopy

Handbook of Single Molecule Fluorescence Spectroscopy PDF
Author: Chris Gell
Publisher: OUP Oxford
ISBN: 9780199673841
Size: 75.86 MB
Format: PDF, ePub, Mobi
Category : Science
Languages : en
Pages : 280
View: 7687

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This book is aimed at providing a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function. The techniques have wide applications in biology, and underpin some aspects of nanotechnology and quantum information processing.

Single Molecule Biophysics And Poisson Process Approach To Statistical Mechanics

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics PDF
Author: Susanta K Sarkar
Publisher: Morgan & Claypool Publishers
ISBN: 1681741806
Size: 20.51 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 74
View: 2156

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This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.

Handbook Of Molecular Biophysics

Handbook of Molecular Biophysics PDF
Author: Henrik G. Bohr
Publisher: Wiley-VCH
ISBN: 9783527407026
Size: 42.47 MB
Format: PDF
Category : Science
Languages : en
Pages : 1074
View: 3268

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This handbook and reference condenses the biophysics and biomedical contents of the renowned Encyclopedia of Applied Physics in one handy volume. Twenty-eight carefully written overview articles cover the latest research, including single molecule spectroscopy, biosensors and cellular biomechanics. Readers benefit from concise summaries of the fundamentals, methods and applications, backed by detailed tables of contents for quick access, and glossaries of terms, as well as detailed lists of references and further reading. For libraries and R&D teams in academia and industry.

Handbook Of Molecular Force Spectroscopy

Handbook of Molecular Force Spectroscopy PDF
Author: Aleksandr Noy
Publisher: Springer Science & Business Media
ISBN: 038749989X
Size: 41.46 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 300
View: 3860

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Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Fluorescence Fluctuation Spectroscopy Ffs

Fluorescence Fluctuation Spectroscopy  FFS  PDF
Author:
Publisher: Academic Press
ISBN: 0123884233
Size: 21.27 MB
Format: PDF, Docs
Category : Science
Languages : en
Pages : 336
View: 2624

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.

Single Molecule Cellular Biophysics

Single Molecule Cellular Biophysics PDF
Author: Mark C. Leake
Publisher: Cambridge University Press
ISBN: 1139619349
Size: 15.47 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages :
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Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.

Biophysical Journal

Biophysical Journal PDF
Author:
Publisher:
ISBN:
Size: 50.86 MB
Format: PDF, Docs
Category : Biophysics
Languages : en
Pages :
View: 7112

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Handbook Of Membrane Channels

Handbook of Membrane Channels PDF
Author:
Publisher: Academic Press
ISBN: 032313890X
Size: 36.70 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 591
View: 868

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This handbook provides a thorough account of recent directions in membrane channel research. Each subject is covered in terms of channel biophysics, pharmacology, and molecular biology. The introductory chapter reviews methodologies of molecular biology currently used for studying molecular structure and function of membrane channels and specific domains in channel proteins.

Handbook Of Dairy Foods Analysis

Handbook of Dairy Foods Analysis PDF
Author: Leo M.L. Nollet
Publisher: CRC Press
ISBN: 1420046314
Size: 26.90 MB
Format: PDF, Mobi
Category : Technology & Engineering
Languages : en
Pages : 918
View: 714

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Dairy foods account for a large portion of the Western diet, but due to the potential diversity of their sources, this food group often poses a challenge for food scientists and their research efforts. Bringing together the foremost minds in dairy research, Handbook of Dairy Foods Analysis compiles the top dairy analysis techniques and methodologies from around the world into one, well-organized volume. Co-Edited by Fidel Toldra - Recipient of the 2010 Distinguished Research Award from the American Meat Science Association Exceptionally comprehensive both in its detailing of methods and the range of products covered, this handbook includes tools for analyzing chemical and biochemical compounds and also bioactive peptides, prebiotics, and probiotics. It describes noninvasive chemical and physical sensors and starter cultures used in quality control. Covers the Gamut of Dairy Analysis Techniques The book discusses current methods for the detection of microorganisms, allergens, and other adulterations, including those of environmental origin or introduced during processing. Other methodologies used to evaluate color, texture, and flavor are also discussed. Written by an International Panel of Distinguished Contributors Under the editorial guidance of renowned authorities, Leo M.L. Nollet and Fidel Toldrá, this handbook is one of the few references that is completely devoted to dairy food analysis – a extremely valuable reference for those in the dairy research, processing, and manufacturing industries.

Dna Topoisomerases

DNA Topoisomerases PDF
Author: Duncan J. Clarke
Publisher: Humana
ISBN:
Size: 60.15 MB
Format: PDF, ePub
Category : Medical
Languages : en
Pages : 267
View: 7697

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DNA topoisomerases and gyrases have been studied thoroughly from a biochemical perspective, and expanding research at the molecular and cellular level shows great promise. This book gives a compendium of protocols that extends into such new areas of research.

Handbook Of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy PDF
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1461571332
Size: 32.75 MB
Format: PDF, Mobi
Category : Medical
Languages : en
Pages : 232
View: 6166

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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Fundamental Concepts In Biophysics

Fundamental Concepts in Biophysics PDF
Author: Thomas Jue
Publisher: Springer Science & Business Media
ISBN: 1597453978
Size: 26.82 MB
Format: PDF
Category : Science
Languages : en
Pages : 248
View: 5496

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In the first volume, Fundamental Concepts in Biophysics, the authors lay down a foundation for biophysics study. Rajiv Singh opens the book by pointing to the central importance of “Mathematical Methods in Biophysics”. William Fink follows with a discussion on “Quantum Mechanics Basic to Biophysical Methods”. Together, these two chapters establish some of the principles of mathematical physics underlying many biophysics techniques. Because computer modeling forms an intricate part of biophysics research, Subhadip Raychaudhuri and colleagues introduce the use of computer modeling in “Computational Modeling of Receptor–Ligand Binding and Cellular Signaling Processes”. Yin Yeh and coworkers bring to the reader’s attention the physical basis underlying the common use of fluorescence spectroscopy in biomedical research in their chapter “Fluorescence Spectroscopy”. Electrophysiologists have also applied biophysics techniques in the study of membrane proteins, and Tsung-Yu Chen et al. explore stochastic processes of ion transport in their “Electrophysiological Measurements of Membrane Proteins”. Michael Saxton takes up a key biophysics question about particle distribution and behavior in systems with spatial or temporal inhomogeneity in his chapter “Single–Particle Tracking”. Finally, in “NMR Measurement of Biomolecule Diffusion”, Thomas Jue explains how magnetic resonance techniques can map biomolecule diffusion in the cell to a theory of respiratory control. This book thus launches the Handbook of Modern Biophysics series and sets up for the reader some of the fundamental concepts underpinning the biophysics issues to be presented in future volumes.

Handbook Of Analysis Of Oligonucleotides And Related Products

Handbook of Analysis of Oligonucleotides and Related Products PDF
Author: Jose V. Bonilla
Publisher: CRC Press
ISBN: 1439819939
Size: 49.50 MB
Format: PDF
Category : Medical
Languages : en
Pages : 511
View: 1324

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Oligonucleotides represent one of the most significant pharmaceutical breakthroughs in recent years, showing great promise as diagnostic and therapeutic agents for malignant tumors, cardiovascular disease, diabetes, viral infections, and many other degenerative disorders. The Handbook of Analysis of Oligonucleotides and Related Products is an essential reference manual on the practical application of modern and emerging analytical techniques for the analysis of this unique class of compounds. A strong collaboration among thirty leading analytical scientists from around the world, the book provides readers with a comprehensive overview of the most commonly used analytical techniques and their advantages and limitations in assuring the identity, purity, quality, and strength of an oligonucleotide intended for therapeutic use. Topics discussed include: Strategies for enzymatic or chemical degradation of chemically modified oligonucleotides toward mass spectrometric sequencing Purity analysis by chromatographic or electrophoretic methods, including RP-HPLC, AX-HPLC, HILIC, SEC, and CGE Characterization of sequence-related impurities in oligonucleotides by mass spectrometry and chromatography Structure elucidation by spectroscopic methods (IR, NMR, MS) as well as base composition and thermal melt analysis (Tm) Approaches for the accurate determination of molar extinction coefficient of oligonucleotides Accurate determination of assay values Assessment of the overall quality of oligonucleotides, including microbial analysis and determination of residual solvents and heavy metals Strategies for determining the chemical stability of oligonucleotides The use of hybridization techniques for supporting pharmacokinetics and drug metabolism studies in preclinical and clinical development Guidance for the presentation of relevant analytical information towards meeting current regulatory expectations for oligonucleotide therapeutics This resource provides a practical guide for applying state-of-the-art analytical techniques in research, development, and manufacturing settings.